Characterization of the Bambusa tulda Roxb inflorescences and flowers at the morphological anatomical and genetic levels to understand floral organ developments and differentiation in bamboo

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dc.contributor.advisorDas, Malay
dc.creator.researcherChakraborty, Sukanya
dc.date.accessioned2024-07-23T06:20:31Z
dc.date.available2024-07-23T06:20:31Z
dc.description.abstractBamboos belong to the monocotyledonous plant family Poaceae and subfamily Bambusoideae. Bamboos represent an extreme example of perennialism, where the reproductive phase begins after completion of an extended vegetative phase, which may vary across species. Other than delayed flowering time, existence of two different types of inflorescences, genetics of floral development and nature of pollen compatibility make bamboo unique in comparison to other grasses. The main aim of this study was to understand the floral organ development and pollination mechanism in Bambusa tulda. In this study, the relative abundance of two different types of inflorescence in an entire flowering cycle were analysed and rate of seed setting was compared. One potential limitation to perform gene expression analyses on Bambusa members was the absence of suitable reference genes to normalize expression data. In this study, reference genes were identified, which were stable across species, organs and developmental stages. In order to understand the molecular mechanism that regulates flower development, the floral meristem identity genes MADS14, MADS15 and MADS18 were sequenced from B. tulda and their expression pattern was analyzed across different vegetative and reproductive tissues. In order to understand the genetics of pollen compatibility in bamboo, in vivo pollination experiments were performed. The finding indicated that B. tulda is primarily a cross pollinated species. In order to further understand the molecular mechanism regulating genetic compatibility in bamboo, selected members of RNase T2 and Fbox gene families were studied. Overall, the findings of this study will be useful to understand the morphological, ecological and molecular basis of flower development and genetic compatibility in bamboos.en_US
dc.description.searchVisibilitytrueen_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.urihttps://www.presiuniv.ac.inen_US
dc.identifier.urihttp://www.presiuniv.ndl.iitkgp.ac.in/handle/123456789/2414
dc.language.isoengen_US
dc.rights.accessRightsauthorizeden_US
dc.sourcePresidency Universityen_US
dc.source.urihttps://www.presiuniv.ac.inen_US
dc.subjectBambooen_US
dc.subjectFloweringen_US
dc.subjectFloral organogenesisen_US
dc.subjectCross pollinationen_US
dc.subjectSelf-incompatibilityen_US
dc.subjectMADS box geneen_US
dc.subjectAPETALA1en_US
dc.titleCharacterization of the Bambusa tulda Roxb inflorescences and flowers at the morphological anatomical and genetic levels to understand floral organ developments and differentiation in bambooen_US
dc.typetexten_US
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