Regulation of Mitochondrial Bioenergetics and Apoptosis Induction In T Cell Lymphomas
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Tumor cells have evolved strategies to down regulate proteins primarily involved in the induction of apoptosis. Since mitochondria play a key role in the regulation of apoptosis identification of novel mitochondrial proteins that programs the tumor cells to endorse the apoptotic pathway is urgently required to develop new anti-cancer therapies and better prognosis of more aggressive forms of cancer like T cell lymphomas. SARM holds such a promise and a detailed mechanistic insight of SARM regulation at the mitochondria is the major goal of this study. How this single-molecule SARM regulates the mitochondrial proteome that leads to excess ROS generation? How SARM regulates mitochondrial bioenergetics that skews the cells to the apoptotic pathway and how this regulation is deranged in cancer. The goal of this proposal is to robustly investigate this novel regulation of mitochondrial energy metabolism by SARM and how this pathway is altered in cancer with emphasis on T cell lymphomas.
The primary objectives of this project were:
Aim1: To examine the role of SARM in cellular apoptosis in nasal NK/T cell lymphoma (NNKTL)
Aim2: To understand the regulation of mitochondrial bioenergetics by SARM leading to cellular apoptosis in NNKTL
Aim3: To identify SARM interacting partners at the mitochondria in NNKTL
n T Cell Lymphomas
Keywords
Cell treatment, Culture and maintenance of SHSY5Y,DAPI and Mitotracker Red staining